A biofuel is tricky to define because the usual fossil fuel we use, is in a way biofuel too. But we can safely say that most of the biofuels don’t add up their quota of carbon dioxide to the environment. The biofuels are therefore considered to be “CO2 neutral.” Researchers from the Biotechnology Foundation Laboratories at Thomas Jefferson University have developed a new method to increase the quantity of oil in tobacco leaves. So that oil in tobacco leaves can be utilized as biofuels in future. Their paper was published in Plant Biotechnology Journal which is an online journal.